Distinguishing Individual Lipid Headgroup Mobility and Phase Transitions in Raft-Forming Lipid Mixtures with 31P MAS NMR

• Published in: Biophysical journal Vol. 90; no. 11; pp. 4248 - 4260
• Main Authors: Holland, Gregory P., McIntyre, Sarah K., Alam, Todd M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2006; Elsevier; Biophysical Society
• Subjects: Cholesterol - chemistry; Lipid Bilayers - chemistry; Membrane Fluidity; Membrane Microdomains - chemistry; Nuclear Magnetic Resonance, Biomolecular; Phase Transition; Phosphatidylcholines - chemistry; Spectroscopy, Imaging Other Techniques; Sphingomyelins - chemistry; Spin Labels

A model membrane system composed of egg sphingomyelin (SM), 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC), and cholesterol was studied with static and magic angle spinning 31P NMR spectroscopy. This model membrane system is of significant biological relevance since it is known to form lipid rafts...