Distinguishing Individual Lipid Headgroup Mobility and Phase Transitions in Raft-Forming Lipid Mixtures with 31P MAS NMR
A model membrane system composed of egg sphingomyelin (SM), 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC), and cholesterol was studied with static and magic angle spinning 31P NMR spectroscopy. This model membrane system is of significant biological relevance since it is known to form lipid rafts...
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Published in | Biophysical journal Vol. 90; no. 11; pp. 4248 - 4260 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.06.2006
Elsevier Biophysical Society |
Subjects | |
Online Access | Get full text |
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Summary: | A model membrane system composed of egg sphingomyelin (SM), 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC), and cholesterol was studied with static and magic angle spinning
31P NMR spectroscopy. This model membrane system is of significant biological relevance since it is known to form lipid rafts.
31P NMR under magic angle spinning conditions resolves the SM and DOPC headgroup resonances allowing for extraction of the
31P NMR parameters for the individual lipid components. The isotropic chemical shift, chemical shift anisotropy, and asymmetry parameter can be extracted from the spinning side band manifold of the individual components that form liquid-ordered and liquid-disordered domains. The magnitude of the
31P chemical shift anisotropy and the line width is used to determine headgroup mobility and monitor the gel-to-gel and gel-to-liquid crystalline phase transitions of SM as a function of temperature in these mixtures. Spin-spin relaxation measurements are in agreement with the line width results, reflecting mobility differences and some heterogeneities. It will be shown that the presence of DOPC and/or cholesterol greatly impacts the headgroup mobility of SM both above and below the liquid crystalline phase transition temperature, whereas DOPC displays only minor variations in these lipid mixtures. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE Address reprint requests to Todd M. Alam, E-mail: tmalam@sandia.gov. |
ISSN: | 0006-3495 1542-0086 |
DOI: | 10.1529/biophysj.105.077289 |