Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite‐incorporated Gold Nanoparticles

• Published in: Electroanalysis (New York, N.Y.) Vol. 34; no. 9; pp. 1519 - 1528
• Main Authors: Yola, Bahar Bankoğlu, Karaman, Ceren, Özcan, Nermin, Atar, Necip, Polat, İlknur, Yola, Mehmet Lütfi
• Format: Journal Article
• Language: English
• Published: 01.09.2022
• Subjects: Alzheimer's disease; Detection; Diagnosis; Nanocomposite; Voltammetry
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.202200159

Herein, electrochemical tau protein immunosensor based on manganese sulfide nanoparticles/graphene oxide/polyaniline (MnS/GO/PANI) and magnetite‐incorporated gold nanoparticles (AuNPs@Fe3O4) was constructed. After the modification of the glassy carbon electrode (GCE) with MnS/GO/PANI, the immobilization and conjugation of anti‐Tau capture antibody and antigen Tau protein were successfully completed on MnS/GO/PANI/GCE, respectively. Then, the conjugation of anti‐Tau secondary antibody was carried out to AuNPs@Fe3O4 as signal amplification via amino‐gold affinity and the eventual immunosensor was accomplished by the distinctive interactions of electrode platform and signal amplification. Final immunosensor demonstrated the quantification limit (LOQ) of 1.0×10−13 M and the detection limit (LOD) of 1.0×10−14 M.