Antibacterial activity and in vivo wound healing potential of phenolic extracts from jaboticaba skin

• Published in: Chemical biology & drug design Vol. 92; no. 1; pp. 1333 - 1343
• Main Authors: Machado, Gustavo Henrique Andrade, Marques, Tamara Rezende, Carvalho, Thaís Cristina Lima, Duarte, André Carvalho, Oliveira, Flávia Cíntia, Gonçalves, Michelle Carlota, Piccoli, Roberta Hilsdorf, Corrêa, Angelita Duarte
• Format: Journal Article
• Language: English
• Published: England 01.07.2018
• Subjects: antimicrobial; HPLC; Plinia jaboticaba; wound healing; antimicrobial; wound healing; HPLC; Plinia jaboticaba
• ISSN: 1747-0277; 1747-0285
• DOI: 10.1111/cbdd.13198

In this study, different phenolic extracts were obtained from the jaboticaba skin meal (JSM), whose phenolic compounds were characterized and their antibacterial activities were assessed. Moreover, the activity of lyophilized ethanolic extract of jaboticaba skin (EEJS) on wound healing was analyzed in rats. The JSM phenolic extracts were obtained in four ways: aqueous, methanolic, ethanolic, and acetone extracts. The phenolic compounds were characterized in these extracts by high‐performance liquid chromatography, and their antibacterial activities were evaluated. The in vivo experiment was divided into four groups and received the following treatments: G1—silver sulfadiazine (positive control); G2—EEJS at 10%; G3—EEJS at 5%, and G4—EEJS at 2.5%. The aqueous extract did not inhibit the growing of any bacterium. The ethanolic, acetone, and methanolic extracts inhibited the growing of all bacteria tested at the concentrations of 1.25%, 2.50%, and 5.00%, respectively. The ethanolic extract was the one that showed the highest bacterial inhibition potential and the highest contents of phenolic compounds, especially of catechin, epicatechin gallate, and epicatechin. The G3 and G4 treatments presented faster wound healing compared to the G1 one, as it promoted a less intense inflammatory reaction and full closure of the wounds at an accelerated rate. In this study, different phenolic extracts were obtained from the jabuticaba skin meal (JSM), whose phenolic compounds were characterized and their antibacterial activities were assessed. Moreover, the activity of lyophilized ethanolic extract of jabuticaba skin (EEJS) on wound healing was analyzed in rats.