β2-Adrenergic receptor stimulation inhibits LPS-induced IL-18 and IL-12 production in monocytes

• Published in: Immunology letters Vol. 101; no. 2; pp. 168 - 172
• Main Authors: Mizuno, Kenji, Takahashi, Hideo Kohka, Iwagaki, Hiromi, Katsuno, Goutaro, Kamurul, Huda A.S.M., Ohtani, Satoru, Mori, Shuji, Yoshino, Tadashi, Nishibori, Masahiro, Tanaka, Noriaki
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2005
• Subjects: Adrenergic receptor; IL-12; IL-18; Adrenergic receptor; IL-12; IL-18
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2005.05.008

We examined the effects of β2-adrenergic receptor (β2-AR) agonists on monocyte-derived cytokines, interleukin (IL)-18 and IL-12 production in lipopolysaccharide (LPS)-stimulated monocytes derived from human peripheral blood mononuclear cells (PBMCs), as in vitro model of sepsis. The study found that β2-AR agonists inhibited IL-18 and IL-12 production in monocytes, and that AR agonist activity was antagonized by the selective β2-AR antagonist, butoxamine. The selective β2-AR agonists salbutamol and terbutaline induced a similar inhibitory pattern of IL-18 and IL-12 production. IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production. Therefore, the stimulation of β2-AR might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.