Studies on the oligomeric structure of yeast phosphofructokinase by means of cross-linking diimidoesters

Intracellular cross-linking of yeast phosphofructokinase with a series of diimidoesters of different chain length resulted in the appearance of tetramers as largest cross-linked product of the enzyme subunits. The native enzyme is evidently composed of eight subunits being arranged in two tetramers...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 71; no. 1; pp. 371 - 378
Main Authors Kopperschläger, Gerhard, Usbeck, Elke, Hofmann, Eberhard
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1976
Subjects
plant biochemistry
plant physiology
SDS
Online AccessGet full text

Cover

More Information
Summary:Intracellular cross-linking of yeast phosphofructokinase with a series of diimidoesters of different chain length resulted in the appearance of tetramers as largest cross-linked product of the enzyme subunits. The native enzyme is evidently composed of eight subunits being arranged in two tetramers α 4β 4. In the tetramers the monomers are probably assembled in tetrahedral geometry.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(76)90292-8