Tests of a practical visible-NIR imaging Fourier transform spectrometer for biological and chemical fluorescence emission measurements

An imaging Fourier transform spectrometer (IFTS) designed for fluorescence emission measurements is reported. The spectral range extension from NIR to visible of the system is realized by using a simple and low-cost optical beam-folding position-tracking technique. Spectral resolution as high as 9.7...

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Bibliographic Details
Published inOptics express Vol. 17; no. 23; pp. 21083 - 21090
Main Authors Li, Jianping, Chan, Robert K Y, Wang, Xuzhu
Format Journal Article
LanguageEnglish
Published United States 09.11.2009
Subjects
Electrons
Equipment Design
Fluorescent Dyes - pharmacology
Fourier Analysis
Infrared Rays
Interferometry - instrumentation
Interferometry - methods
Oligonucleotide Array Sequence Analysis - instrumentation
Optics and Photonics - instrumentation
Optics and Photonics - methods
Protein Array Analysis - instrumentation
Quantum Dots
Spectrometry, Fluorescence - methods
Spectroscopy, Fourier Transform Infrared - methods
Spectroscopy, Near-Infrared
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Summary:An imaging Fourier transform spectrometer (IFTS) designed for fluorescence emission measurements is reported. The spectral range extension from NIR to visible of the system is realized by using a simple and low-cost optical beam-folding position-tracking technique. Spectral resolution as high as 9.78cm(-1)(0.4nm at 632.8nm) and maximum image resolution up to 300x300 pixels are proved by the system tests on its optical performances. Imaging fluorescence spectra acquisition of quantum dot clusters and single 200nm diameter fluorescent beads have demonstrated the system's potential for high throughput imaging spectroscopic measurements of fluorescent biological and chemical samples.
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content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.17.021083