Flocculation in Schizosaccharomyces pombe

• Published in: Journal of general microbiology Vol. 64; no. 2; pp. 247 - 250
• Main Author: CALLEJA, G. B
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.12.1970
• Subjects: Ascomycota - drug effects; Ascomycota - growth & development; Ascomycota - physiology; Calcium - pharmacology; Cell Aggregation; Temperature
• ISSN: 0022-1287
• DOI: 10.1099/00221287-64-2-247

Biochemistry Laboratory, National Research Council of Canada, Ottawa, Canada ABSTRACT SUMMARY: Spontaneous formation of macroscopic aggregates of yeast cells is termed flocculation. Many papers have been written on the subject (for reviews see Comrie, 1952, Gilliland, 1957; Jansen, 1958; Rainbow, 1966; Windisch, 1968), but because of the importance of the phenomenon to the brewer most of the studies have been confined to brewer's yeast. An analogous phenomenon in a fission yeast is described here. Schizosaccharomyces pombe NCYC 132 was grown in 125 ml. Erlenmeyer flasks each containing 10 ml. malt-extract broth (Oxoid, 2%, w/v). The inoculum consisted of 5 x 10 6 stationary-phase yeast cells from a 24 h. static culture in the same medium in a tightly capped McCartney bottle. Cultures were shaken on a rotatory shaker at 150 rev./min. at 32°; the generation time was 120 min.