Clinical experimental study of GoldMag® immunochromatography in high sensitive C reactive protein detection from whole blood and plasma

• Published in: Journal of magnetism and magnetic materials Vol. 473; pp. 68 - 73
• Main Authors: Zhang, Lixia, Zhang, Qinlu, Gao, Min, Luo, Zhiyi, Zhang, Ying, Li, Xiaoxia, Hua, Kai, Zhang, Chao, Lai, Wenting, Cui, Yali
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.03.2019; Elsevier BV
• Subjects: Blood; Blood groups; Correlation analysis; GoldMag® immunochromatography; hs-CRP; Immunoassay; Immunotherapy; Nanoparticles; Patients; Plasma; Proteins; Scattering; Turbidimetry; Correlation analysis; hs-CRP; GoldMag® immunochromatography
• ISSN: 0304-8853; 1873-4766
• DOI: 10.1016/j.jmmm.2018.09.129

•The GoldMag® immunochromatography provide results more stable.•The sensitivity can reach picogram level regardless whole blood and plasma specimens.•This method especially suitable for township hospitals and community.•The assay provides faster and more accurate results for clinical diagnosis. To investigate the consistency between GoldMag® immunochromatography and immune scatter turbidimetry in hs-CRP detection. The data of hs-CRP were collected from 78 hospitalized patients, and these patients were divided into fingertip blood group and the venous blood group according to the different blood collection methods. The whole blood and plasma level of hs-CRP was detected by GoldMag® immunochromatography and immune scatter turbidimetry. Correlation analysis was carried out to compare the data of hs-CRP obtained by different approaches. In both fingertip blood group and venous blood group, the concentration of hs-CRP determined by GoldMag® immunochromatography were positively correlated with those found with immune scatter turbidimetry. There was no significant difference in the level of hs-CRP provided by these two different approaches. (P > 0.05). GoldMag® immunochromatographic assay has good uniformity with immune scatter turbidimetry Scatter Turbidimetric Method for hs-CRP detection. Based on the double antibody sandwich method, antibody labeled GoldMag nanoparticles capture hs-CRP from whole blood or plasma and aggregate in test line of test strip. The magnetic intensity of agglomerated GoldMag® nanoparticles collected is measured by magnetic quantitative immunoassay analyzer. The concentration of hs-CRP can be obtained according to the standard curve between concentration and magnetic intensity. GoldMag® immunochromatography is served as an easy operation, high accuracy and stable method.