Process Performance and Bacterial Community Structure Under Increasing Influent Disturbances in a Membrane-Aerated Biofilm Reactor

• Published in: Journal of microbiology and biotechnology Vol. 26; no. 2; pp. 373 - 384
• Main Authors: Tian, Hailong, Yan, Yingchun, Chen, Yuewen, Wu, Xiaolei, Li, Baoan
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.02.2016
• Subjects: Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Biofilms; Biological Oxygen Demand Analysis; Bioreactors; Carbon - metabolism; Microbial Consortia - physiology; Nitrogen - metabolism; Nitrosomonas - genetics; Nitrosomonas - physiology; Oxygen - metabolism; Principal Component Analysis; Waste Disposal, Fluid - standards; Waste Water - chemistry; 생물학; flow velocity; bacterial community assembly; MABR; biofilm
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1506.06072

The membrane-aerated biofilm reactor (MABR) is a promising municipal wastewater treatment process. In this study, two cross-flow MABRs were constructed to explore the carbon and nitrogen removal performance and bacterial succession, along with changes of influent loading shock comprising flow velocity, COD, and NH4-N concentrations. Redundancy analysis revealed that the function of high flow velocity was mainly embodied in facilitating contaminants diffusion and biosorption rather than the success of overall bacterial populations (p > 0.05). In contrast, the influent NH4-N concentration contributed most to the variance of reactor efficiency and community structure (p < 0.05). Pyrosequencing results showed that Anaerolineae, and Beta- and Alphaproteobacteria were the dominant groups in biofilms for COD and NH4-N removal. Among the identified genera, Nitrosomonas and Nitrospira were the main nitrifiers, and Hyphomicrobium, Hydrogenophaga, and Rhodobacter were the key denitrifiers. Meanwhile, principal component analysis indicated that bacterial shift in MABR was probably the combination of stochastic and deterministic processes.