α-Glucan synthesis in the cotyledons of germinating lupins

On germination, both in the dark and light, a glucan, that can be extracted with DMSO and precipitated as the I 2 complex, is synthesized in the cotyledons of lupins. In the dark, it can be labelled with radioactivity from U- 13C-labelled d-glucose, d-fructose, d-galactose, l-arabinose and sucrose....

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Bibliographic Details
Published inPhytochemistry (Oxford) Vol. 20; no. 4; pp. 641 - 645
Main Authors Saini, H.S., Matheson, N.K.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 1981
Subjects
cotyledons
germination
Leguminosae
lupins
Lupinus lupeus
plant biochemistry
plant physiology
starch
α-glucan
germination
Lupinus lupeus
Leguminosae
α-glucan
lupins
starch
cotyledons
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Summary:On germination, both in the dark and light, a glucan, that can be extracted with DMSO and precipitated as the I 2 complex, is synthesized in the cotyledons of lupins. In the dark, it can be labelled with radioactivity from U- 13C-labelled d-glucose, d-fructose, d-galactose, l-arabinose and sucrose. Enzymic hydrolysis of this material was consistent with it being α (1 → 4) (1 → 6) glucan. The iodine absorption spectra and gel chromatographic behaviour on Sepharose CL-2B of extracts at 4 days and later after imbibition indicated that the polysaccharide was starch-like. The amylose percentage increased with time of germination. The material extracted at 2 days contained no significant amount of amylose and the gel chromatographic behaviour differed somewhat from that of amylopectin.
ISSN:0031-9422
1873-3700
DOI:10.1016/0031-9422(81)85149-7