Characterization of macrophage cytotoxicity factor (MCF)

Allogenetically sensitized T lymphocytes secrete in the presence of the specific antigen a factor which renders macrophages cytotoxic to target cells in vitro, macrophage cytotoxicity factor. Cytotoxicity is expressed as 51Cr release from labeled target cells. Experiments are described to characteri...

Full description

Saved in:
Bibliographic Details
Published inInternational archives of allergy and applied immunology Vol. 53; no. 1; p. 37
Main Authors Hall, N D, Lohmann-Matthes, M L, Knüfermann, H, Kickhöfen, B
Format Journal Article
LanguageEnglish
Published Switzerland 1977
Subjects
Animals
Chromatography
Cytotoxicity Tests, Immunologic
In Vitro Techniques
Macrophages - immunology
Mice
T-Lymphocytes - analysis
Online AccessGet more information

Cover

More Information
Summary:Allogenetically sensitized T lymphocytes secrete in the presence of the specific antigen a factor which renders macrophages cytotoxic to target cells in vitro, macrophage cytotoxicity factor. Cytotoxicity is expressed as 51Cr release from labeled target cells. Experiments are described to characterize chemically nonspecific MCF activity. After purification on a DEAE-cellulose column, active material elutes from Sephadex G-100 in a single peak. Assaying individual fractions of this peak shows that nonspecific MCF activity is found at a mol. wt. of 28,000+/-5,000. This material is stable at -70 degrees C several months.
ISSN:0020-5915
DOI:10.1159/000231728