Determination of warfarin in animal relicta and feedingstuffs by high-pressure liquid chromatography with confirmation of identity by mass spectrometry

The determination of warfarin in liver, blood, urine and feedingstuffs by high-pressure liquid chromatography (HPLC) is described. Clean-up is simple and the lower limit of determination is about 0.025 ppm. Recoveries from liver are about 85% over the range 0.025-1.0 ppm; recoveries from blood range...

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Bibliographic Details
Published inJournal of chromatography Vol. 121; no. 2; p. 335
Main Authors Mundy, D E, Quick, M P, Machin, A F
Format Journal Article
LanguageEnglish
Published Netherlands 23.06.1976
Subjects
Animal Feed - analysis
Animals
Chromatography, High Pressure Liquid
Liver - analysis
Mass Spectrometry
Methods
Microchemistry
Warfarin - analysis
Warfarin - blood
Warfarin - urine
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Summary:The determination of warfarin in liver, blood, urine and feedingstuffs by high-pressure liquid chromatography (HPLC) is described. Clean-up is simple and the lower limit of determination is about 0.025 ppm. Recoveries from liver are about 85% over the range 0.025-1.0 ppm; recoveries from blood range from about 67 to 90%. Chemical ionisation mass spectrometry can be used to confirm identity and, by specific ion monitoring, to detect warfarin in HPLC eluates at levels below the sensitivity of the HPLC detector.
DOI:10.1016/S0021-9673(00)85031-1