Repeatability and Reproducibility of Quantitative Assessment of the Retinal Microvasculature Using Optical Coherence Tomography Angiography Based on Optical Microangiography

• Published in: Biomedical and environmental sciences Vol. 31; no. 6; pp. 407 - 412
• Main Authors: ZHAO, Qi, YANG, Wen Li, WANG, Xiao Na, Wang, Ruikang K, YOU, Qi Sheng, CHU, Di Zhong, XIN, Chen, ZHANG, Meng Yu, LI, Dong Jun, WANG, Zi Yang, CHEN, Wei, LI, Yi Feng, CUI, Rui, SHEN, Lin, WEI, Wen Bin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.06.2018; Department of Bioengineering,University of Washington,Seattle Washington 98195,United States of America%Department of Bioengineering,University of Washington,Seattle Washington 98195,United States of America; Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100005,China; Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100730,China%Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University; Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100005,China%Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University; Department of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University
• Subjects: Macular blood flow; Optical coherence tomography angiography; Repeatability; Reproducibility; Retinal superficial capillary; Vascular quantification; Repeatability; Retinal superficial capillary; Vascular quantification; Optical coherence tomography angiography; Reproducibility; Macular blood flow
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2018.054

The aim of this study was to determine the repeatability and reproducibility of optical coherence tomography angiography (OCTA) based on optical microangiography (OMAG) measurements of macular vessels in normal eyes. In this prospective cohort study, 40 eyes of 40 healthy volunteers underwent repeated OCTA (Cirrus HD-OCT 5000 angiography system, Carl Zeiss Meditec, Inc.) scans on two separate visit days. On each visit day, the eyes were scanned three times. The following parameters were used to quantitatively describe the OCTA images of the superficial vascular network: vessel area density (VAD), vessel skeleton density (VSD), vessel diameter index (VDI), vessel perimeter index (VPI), vessel complexity index (VCI), flux, and foveal avascular zone (FAZ). Coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated for evaluating intravisit and intervisit repeatability, as well as interobserver reproducibility. The measurements showed high repeatability [CVs ≤ 4.2% (intravisit) and ≤ 4.6% (intervisit)] and interobserver reproducibility (ICCs ≥ 0.923) for all parameters. This study demonstrated good repeatability and reproducibility of OCTA based on OMAG for the measurement of superficial vessel parameters in normal eyes.