13C Natural Isotope Abundance in Urothelium as a New Marker in the Follow-Up of Patients with Bladder Cancer

Bladder cancer (BC) is the most common urological malignancy and has a high incidence of recurrence. BC cells alter their nutrient uptake and metabolic pathways in order to continue the production of sufficient levels of ATP and metabolic intermediates for proliferation and survival. Changes in meta...

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Published inCancers Vol. 14; no. 10; p. 2423
Main Authors Madej, Adam, Forma, Ewa, Golberg, Michał, Kamiński, Rafał, Paneth, Piotr, Kobos, Józef, Różański, Waldemar, Lipiński, Marek
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 13.05.2022
MDPI
Subjects
Biopsy
Bladder cancer
Cancer
Carbon
Cellular biology
Hematuria
Intermediates
Invasiveness
Isotopes
Malignancy
Mass spectrometry
Mass spectroscopy
Medical prognosis
Metabolic pathways
Metabolism
Nitrogen
Nutrient uptake
Patients
Scientific imaging
Statistical analysis
Sulfur
Tumors
Urogenital system
Urothelium
United Kingdom > UK
England
Poland
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Summary:Bladder cancer (BC) is the most common urological malignancy and has a high incidence of recurrence. BC cells alter their nutrient uptake and metabolic pathways in order to continue the production of sufficient levels of ATP and metabolic intermediates for proliferation and survival. Changes in metabolic pathways regarding the rate of the enzymatic reaction and transport lead to differences in the content of natural isotopes (13C, 15N, 34S) between normal and cancerous tissues. The assessment of the stable isotopes of carbon, nitrogen, and sulfur in normal urothelium and bladder cancer samples was performed using Isotope Ratio Mass Spectrometry (IRMS). The natural abundance of 15N and 13C was decreased in bladder cancer samples when compared to normal urothelium. No significant correlation was observed in BC specimens depending on the tumor grade and stage. Samples derived from bladder tumors and normal urothelium had a different pattern of 15N and 13C isotope abundance. Decreased 13C natural isotopes in the normal urothelium of BC patients were significantly associated with a shorter DFS. Our results suggest that isotopic analysis of normal urothelium of BC patients can be used to predict bladder cancer recurrence.
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ISSN:2072-6694
2072-6694
DOI:10.3390/cancers14102423