Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation

• Published in: Neural regeneration research Vol. 7; no. 4; pp. 251 - 255
• Main Authors: Kang, Huicong, Hu, Qi, Liu, Xiaoyan, Liu, Yinhe, Xu, Feng, Li, Xiang, Zhu, Suiqiang
• Format: Journal Article
• Language: English
• Published: India Department of Neurology, Tongji Hospital of Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China%Ministry of Water Resources of China, Beijing 100000, China 05.02.2012; Medknow Publications & Media Pvt Ltd
• Subjects: Research and Report: Stem Cells and Neural Regeneration; 免疫印迹; 免疫荧光; 受体表达; 干细胞移植; 海马CA3区; 系统; 腺苷; 骨髓; cell transplantation; adenosine system; reconstruction; immunohistochemistry; bone marrow-derived mesenchymal stem cells; chronic epilepsy; electroencephalogram
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.04.002

In the present study, we transplanted bone marrow-derived mesenchymal stem cells into the CA3 area of the hippocampus of chronic epilepsy rats kindled by lithium chloride-pilocarpine, Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression and a decrease in adenosine A2a receptor expression in the brain tissues of epileptic rats 3 months after transplantation. Moreover, the imbalance in the A1 adenosine receptor/A2a adenosine receptor ratio was improved. Electroencephalograms showed that frequency and amplitude of spikes in the hippocampus and frontal lobe were reduced. These results suggested that mesenchymal stem cell transplantation can reconstruct the normal function of the adenosine system in the brain and greatly improve epileptiform discharges.