Interference of Helix pomatia extracts on the determination of methandriol in veterinary residue control analysis

• Published in: Analyst (London) Vol. 123; no. 12; pp. 2643 - 2644
• Main Authors: LEYSSENS, L, VAN PUYMBROECK, M, RAUS, J
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.12.1998
• Subjects: Anabolic Agents - urine; Animal productions; Animals; Biological and medical sciences; Buffers; Cattle; Drug Residues - analysis; False Negative Reactions; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; General aspects; Helix (Snails); Methandriol - urine; Methyltestosterone - analysis; Veterinary Drugs - urine; Helicidae; Biological fluid; Helix pomatia; Escherichia coli; Gastropoda; Veterinary medicine; Biological material; Pulmonata; Investigation method; Methandriol; Bacteria; Feces; Enterobacteriaceae; Quantitative analysis; Urine; β-Glucuronidase; Enzyme; Interference; Enzymatic method; Microbial origin; Glycosidases; Hydrolases; Invertebrata; Mollusca; Anabolic agent; O-Glycosidases

Aqueous solutions containing methandriol (MAD) were incubated with Succus helix pomatia (SHP) or beta-glucuronidase from Escherichia coli (EC). SHP, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of MAD into methyltestostero...