Interference of Helix pomatia extracts on the determination of methandriol in veterinary residue control analysis

Aqueous solutions containing methandriol (MAD) were incubated with Succus helix pomatia (SHP) or beta-glucuronidase from Escherichia coli (EC). SHP, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of MAD into methyltestostero...

Full description

Saved in:
Bibliographic Details
Published inAnalyst (London) Vol. 123; no. 12; pp. 2643 - 2644
Main Authors LEYSSENS, L, VAN PUYMBROECK, M, RAUS, J
Format Conference Proceeding Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 01.12.1998
Subjects
Anabolic Agents - urine
Animal productions
Animals
Biological and medical sciences
Buffers
Cattle
Drug Residues - analysis
False Negative Reactions
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
General aspects
Helix (Snails)
Methandriol - urine
Methyltestosterone - analysis
Veterinary Drugs - urine
Helicidae
Biological fluid
Helix pomatia
Escherichia coli
Gastropoda
Veterinary medicine
Biological material
Pulmonata
Investigation method
Methandriol
Bacteria
Feces
Enterobacteriaceae
Quantitative analysis
Urine
β-Glucuronidase
Enzyme
Interference
Enzymatic method
Microbial origin
Glycosidases
Hydrolases
Invertebrata
Mollusca
Anabolic agent
O-Glycosidases
Online AccessGet full text

Cover

More Information
Summary:Aqueous solutions containing methandriol (MAD) were incubated with Succus helix pomatia (SHP) or beta-glucuronidase from Escherichia coli (EC). SHP, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of MAD into methyltestosterone. No conversion occurred when bacterial beta-glucuronidase from E. coli was used.
ISSN:0003-2654
1364-5528
DOI:10.1039/a805018j