Investigation of poly (ADP-ribose) polymerase-1 genetic variants as a possible risk for allergic rhinitis

• Published in: Genetic testing and molecular biomarkers Vol. 18; no. 1; p. 57
• Main Authors: Ozaydin, Ahmet, Akbas, Fahri, Aksoy, Fadlullah, Yildirim, Yavuz Selim, Demirhan, Hasan, Karakurt, Funda, Koc, Arzuhan, Onaran, Ilhan, Kanigur Sultuybek, Gonul
• Format: Journal Article
• Language: English
• Published: United States 01.01.2014
• Subjects: Base Sequence; DNA Primers; Genetic Predisposition to Disease; Humans; Poly(ADP-ribose) Polymerases - genetics; Polymerase Chain Reaction; Rhinitis, Allergic; Rhinitis, Allergic, Perennial - genetics
• ISSN: 1945-0257
• DOI: 10.1089/gtmb.2013.0363

Recent studies point toward the involvement of poly (ADP-ribose) polymerase-1 (PARP-1) in the pathogenesis of allergic airway inflammation, such as asthma and allergic rhinitis (AR). It has been suggested that inhibition of PARP-1 provides significant protection against systemic or tissue inflammation in animal models. The objective of this study was to investigate whether single-nucleotide polymorphisms of PARP-1 gene are associated with genetic susceptibility to AR. We studied the effect of promoter variations and Val762Ala polymorphism of the PARP-1 gene on the risk for developing AR in a case-control association study with 110 RA patients and 130 control subjects in a Turkish population. The polymorphisms of 410 C/T, -1672G/A, and Val762Ala in the PARP-1 gene were analyzed using the polymerase chain reaction-restriction fragment length polymorphism method. Haplotype analysis of these groups was also performed. The results were statistically analyzed by calculating the odds ratio (OR) and their 95% confidence intervals using χ(2) tests. The heterozygote genotype of the promoter polymorphism (-1672) was significantly found to be associated with susceptibility to AR (OR: 0.56) among the tested single-nucleotide polymorphisms. Haplotypes of PARP-1 -410, -1672, and 762 were not associated with an increased risk for AR. These results raise the possibility that the promoter (-1672) polymorphism of the PARP-1 gene may be a risk factor for AR.