Seed surface gums content using the Dubois carbohydrate analysis

The Dubois total carbohydrate method was applied to quantitative analysis of seed surface gums removed from Lesquerella seed by ultrasound. Between 20 and 30 seed samples could be tested in triplicate in a normal 8-h day. The analyses were repeatable with a coefficient of variation of 4%. Germinatio...

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Bibliographic Details
Published inIndustrial crops and products Vol. 4; no. 3; pp. 185 - 192
Main Authors Abbott, Thomas P., Nelsen, Terry C., Kleiman, Robert
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1995
Subjects
carbohydrates
chemical constituents of plants
Gums
Lesquerella
Lesquerella fendleri
Mucilage
Polysaccharide
polysaccharides
quantitative analysis
seed germination
seeds
ultrasonics
Lesquerella fendleri
Mucilage
Polysaccharide
Gums
Lesquerella
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Summary:The Dubois total carbohydrate method was applied to quantitative analysis of seed surface gums removed from Lesquerella seed by ultrasound. Between 20 and 30 seed samples could be tested in triplicate in a normal 8-h day. The analyses were repeatable with a coefficient of variation of 4%. Germination rates of seeds were unaffected by the sonic treatment. Of 16 Lesquerella species tested, L. fendleri had the highest amount of high-molecular-weight gums. Seeds from various Lesquerella species had 4.5 to 17.3% seed surface gum content. Lesquerella fendleri seed presscake (27.8% gum), hexane-defatted meal (35.6% gum) and gums of other genera were also amenable to the test method.
Bibliography:http://hdl.handle.net/10113/25061
ISSN:0926-6690
1872-633X
DOI:10.1016/0926-6690(95)00031-7