Aptamer biosensor for protein detection based on guanine-quenching

Based on fluorescence quenching between guanine bases and fluorophore, a cost-effective biosensor for protein detection is developed. In this biosensor, the label-free protein is detected through variation in fluorescence induced by aptamer conformational change during the recognition between aptame...

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Bibliographic Details
Published inSensors and actuators. B, Chemical Vol. 129; no. 1; pp. 211 - 217
Main Authors Wang, Wenjuan, Chen, Chunlai, Qian, Min Xie, Zhao, Xin Sheng
Format Journal Article
LanguageEnglish
Published Elsevier B.V 29.01.2008
Subjects
Aptamer
Aptamer beacon
Biosensor
Guanine quenching
Protein detection
Thrombin
Thrombin
Aptamer
Guanine quenching
Aptamer beacon
Protein detection
Biosensor
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Summary:Based on fluorescence quenching between guanine bases and fluorophore, a cost-effective biosensor for protein detection is developed. In this biosensor, the label-free protein is detected through variation in fluorescence induced by aptamer conformational change during the recognition between aptamer and protein. Using thrombin-binding aptamer and thrombin as a model system, two design strategies, which are named as hairpin design and duplex design, are developed. Moreover, we investigated how the detection limit is influenced by the modifications, the stem length, and the number of quenching guanine bases in the hairpin design, or the length of the complementary sequence in the duplex design. Our results showed that, to optimize the detection limit, the balance between binding ability of aptamer to protein and quenching efficiency of guanine quenching aptamer have to be achieved.
Bibliography:ObjectType-Article-1
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ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2007.07.125