Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cells

• Published in: Czech Journal of Animal Science Vol. 67; no. 7; pp. 266 - 274
• Main Authors: Huang, Huayu, Zhang, Chen, Feng, Luqiu, Wang, Naixiu, Wang, Kai, Song, Zhenhui, Gan, Ling
• Format: Journal Article
• Language: English; Czech; Slovak
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2022; Czech Academy of Agricultural Sciences
• Subjects: Absorption; amino acid transport system; Amino acids; antioxidant activity; Antioxidants; carboxymethyl modification; Carboxymethylation; Cysteine; Damage; Design optimization; Epithelial cells; Epithelium; Fourier transforms; Hydroxyl radicals; Infrared analysis; intestinal health; Intestine; Oligosaccharides; Oxidative stress; Scavenging; Swine; Transportation systems
• ISSN: 1212-1819; 1805-9309
• DOI: 10.17221/138/2021-CJAS

Oxidative stress is associated with retarded growth and the initiation and progression of diseases in pigs. The carboxymethylation of chito-oligosaccharide (COS) can improve its antioxidant activity for such issues. Herein, an orthogonal experiment L9 (34) test design was used to optimise the preparation conditions of carboxymethyl (CM)-COS. Fourier-transform infrared analysis evidenced the carboxymethylation of COS, and an in vitro study indicated that the hydroxyl radical scavenging ability of CM-COS is superior to that of COS. CM-COS can also better promote the absorption of cysteine and increase the expression of the amino acid transport system b0,+ in intestinal porcine epithelial cells. The results suggested that CM-COS can effectively resist oxidative damage by promoting cysteine absorption mediated by transport system b0,+, which provides important information regarding the antioxidative damage application of COS and CM-COS in the pig farming industry.