Vertical optical sectioning using a magnetically driven confocal microscanner aimed for in vivo clinical imaging

This paper presents a confocal microscanner for direct vertical optical sectioning of biological samples. Confocal imaging is performed by transverse (X-axis) and axial (Z-axis) scanning of a focused laser beam using an optical fiber and a microlens respectively. The actuators are fabricated by lase...

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Bibliographic Details
Published inOptics express Vol. 19; no. 25; pp. 25161 - 25172
Main Authors Mansoor, Hadi, Zeng, Haishan, Chen, Keqin, Yu, Yingqiu, Zhao, Jianhua, Chiao, Mu
Format Journal Article
LanguageEnglish
Published United States 05.12.2011
Subjects
Equipment Design
Equipment Failure Analysis
Lenses
Magnetics - instrumentation
Micro-Electrical-Mechanical Systems - instrumentation
Microscopy, Confocal - instrumentation
Microtomy - instrumentation
Miniaturization
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Summary:This paper presents a confocal microscanner for direct vertical optical sectioning of biological samples. Confocal imaging is performed by transverse (X-axis) and axial (Z-axis) scanning of a focused laser beam using an optical fiber and a microlens respectively. The actuators are fabricated by laser micromachining techniques and are driven by electromagnetic forces. Optical and mechanical performance of the system is predicted by simulation software packages and characterized by experimental measurements. The scanner has lateral resolution of 3.87 µm and axial resolution of 10.68 µm with a field of view of 145 µm in X and 190 µm in Z directions. Confocal imaging of a polymer layer deposited on a silicon wafer and onion epidermal cells is demonstrated.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.19.025161