Design and Implementation of a Miniaturized Dual-wavelength UV Crosslinker

To satisfy the requirement of microarray fabrication in optical sensing applications, we report a palm-sized UV LED crosslinker. By employing a microcontroller and the Wi-Fi communication technique, a sequence of different wavelengths, irradiation intensities, and crosslinking durations can be progr...

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Bibliographic Details
Published inSensors and materials Vol. 34; no. 2; p. 835
Main Authors Wang, Zhiyou, Wang, Maojin
Format Journal Article
LanguageEnglish
Published Tokyo MYU Scientific Publishing Division 01.01.2022
Subjects
Arrays
Crosslinking
Fluorescence
Irradiation
Microcontrollers
Proteins
Substrates
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Summary:To satisfy the requirement of microarray fabrication in optical sensing applications, we report a palm-sized UV LED crosslinker. By employing a microcontroller and the Wi-Fi communication technique, a sequence of different wavelengths, irradiation intensities, and crosslinking durations can be programmed by an Android app. To evaluate the irradiation uniformity of the crosslinker, we immobilized an array of fluorescent-labeled proteins on a silver substrate of high flatness by the reported crosslinker in 8 s and measured the fluorescence intensity throughout the array. For protein spots of the same concentration, the intensity difference is around 10% of the average value. The results show that the reported UV LED crosslinker can help to achieve the uniform and efficient immobilization of a protein microarray.
ISSN:0914-4935
2435-0869
DOI:10.18494/SAM.2022.3644