Photoaffinity labelling of glycosyltransferases

The photoaffinity analogues 5-azido-UDP-glucose and 5-azido-UDP-glucuronic acid have proven to be valuable biochemical tools in the studies of nucleoside diphosphate sugar-utilizing enzymes, especially membrane-associated glycosyltransferases. A summary of the past and current uses of these analogue...

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Bibliographic Details
Published inGlycobiology (Oxford) Vol. 2; no. 4; p. 279
Main Authors Drake, R R, Elbein, A D
Format Journal Article
LanguageEnglish
Published England 01.08.1992
Subjects
Affinity Labels - chemistry
Animals
Azides - chemistry
Binding Sites
Glycosyltransferases - chemistry
Glycosyltransferases - metabolism
Humans
Molecular Structure
Photochemistry
Uridine Diphosphate Glucose - analogs & derivatives
Uridine Diphosphate Glucose - chemistry
Uridine Diphosphate Glucuronic Acid - analogs & derivatives
Uridine Diphosphate Glucuronic Acid - chemistry
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Summary:The photoaffinity analogues 5-azido-UDP-glucose and 5-azido-UDP-glucuronic acid have proven to be valuable biochemical tools in the studies of nucleoside diphosphate sugar-utilizing enzymes, especially membrane-associated glycosyltransferases. A summary of the past and current uses of these analogues is presented, as well as photoaffinity data for the enzyme UDP-glucose: dolichylphosphate glucosyltransferase (Glc-P-Dol synthase). This enzyme has served as a model membrane-associated glycosyltransferase for demonstrating the uses of 5-azido-UDP-glucose. The advantages of using photoaffinity analogues for the purification and characterization of glycosyltransferases are presented, as well as an outline of the general procedures which can be used in conjunction with these analogues.
ISSN:0959-6658
DOI:10.1093/glycob/2.4.279