Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy

Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary imp...

Full description

Saved in:
Bibliographic Details
Published inOptics express Vol. 18; no. 16; pp. 16640 - 16645
Main Authors Kudlinski, A, Lelek, M, Barviau, B, Audry, L, Mussot, A
Format Journal Article
LanguageEnglish
Published United States 02.08.2010
Subjects
Color
Diagnostic Imaging - instrumentation
Equipment Design
Flow Cytometry - methods
Lasers
Microscopy, Fluorescence - instrumentation
Photons
Online AccessGet full text

Cover

More Information
Summary:Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.18.016640