Effect of chronic administration of ethanol on the regulation of the δ-subunit of GABAA receptors in the rat brain

Abstract In the present study, we investigated the effect of chronic ethanol (CE) administration on the polypeptide levels of the δ-subunit of GABAA receptors and [3 H]muscimol binding to the immunoprecipitated δ-subunit-containing GABAA receptor assemblies in the rat brain. CE administration result...

Full description

Saved in:
Bibliographic Details
Published inBrain research Vol. 1174; pp. 47 - 52
Main Authors Marutha Ravindran, C.R, Mehta, Ashok K, Ticku, Maharaj K
Format Journal Article
LanguageEnglish
Published 12.10.2007
Subjects
Neurology
CC
CE
δ-Subunit
Immunoprecipitation
hippocampus
chronic ethanol
cerebellum
Polypeptide levels
cerebral cortex
chronic intermittent ethanol
GABA A receptors
Radioligand binding
HC
CIE
CB
Online AccessGet full text

Cover

More Information
Summary:Abstract In the present study, we investigated the effect of chronic ethanol (CE) administration on the polypeptide levels of the δ-subunit of GABAA receptors and [3 H]muscimol binding to the immunoprecipitated δ-subunit-containing GABAA receptor assemblies in the rat brain. CE administration resulted a down-regulation of polypeptide levels of the δ-subunit of GABAA receptors in the rat cerebellum and hippocampus, whereas there were no changes in the δ-subunit polypeptide levels in the rat cerebral cortex. Further, CE administration caused a down-regulation of native δ-subunit-containing GABAA receptor assemblies in the rat cerebellum as determined by [3 H]muscimol binding to the immunoprecipitated receptor assemblies. These results indicate that the δ-subunit-containing GABAA receptors may play a role in chronic ethanol-induced tolerance and dependence.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2007.07.077