Improved performance of a protein array using conjugated polymers as capture phase for HIV serodiagnosis

A modified recombinant protein derived from the viral capsid of the human immunodeficiency virus (HIV) was coupled to a copolymer of maleic anhydride and methyl vinyl ether (MAMVE). The conjugated copolymer was spotted versus free protein in 96‐well microplates. The conjugated copolymer largely impr...

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Bibliographic Details
Published inPolymer international Vol. 53; no. 5; pp. 586 - 590
Main Authors Perrin, Agnès, Duracher, David, Allard, Laure, Cleuziat, Philippe, Theretz, Alain, Mandrand, Bernard
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 01.05.2004
Subjects
96-well microplate
colorimetry
enzymatic detection
HIV
immunoassay
MAMVE copolymer
oriented coupling
P24
protein microarray
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Summary:A modified recombinant protein derived from the viral capsid of the human immunodeficiency virus (HIV) was coupled to a copolymer of maleic anhydride and methyl vinyl ether (MAMVE). The conjugated copolymer was spotted versus free protein in 96‐well microplates. The conjugated copolymer largely improved detection sensitivity of anti‐P24 antibodies in infected human sera. Results obtained with immuno‐linked sorbent array (ILISA) were correlated with those generated with a standard enzyme‐linked immunosorbent assay (ELISA). Serum dilution dynamic range was varied from 1/5000 to 1/150, and inter‐well coefficient of variation was below 8%. Copyright © 2004 Society of Chemical Industry
Bibliography:istex:0B1D60889B229C54B7A5E09B2E7C83FF6EF5ED7D
ArticleID:PI1436
ark:/67375/WNG-VVN96J5Q-7
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0959-8103
1097-0126
DOI:10.1002/pi.1436