A portion of the Pf155/RESA antigen of Plasmodium falciparum is accessible on the surface of infected erythrocytes
An investigation of antigens accessible to lactoperoxidase-catalysed cell surface iodination on intact Plasmodium falciparum-infected red blood cells (RBC) has identified a 125I-labelled antigen with an apparent size of about 155 kD. This labelled protein was specifically immunoprecipitated by the f...
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Published in | Immunology and cell biology Vol. 66 ( Pt 4); p. 269 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.08.1988
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Subjects | |
Online Access | Get more information |
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Summary: | An investigation of antigens accessible to lactoperoxidase-catalysed cell surface iodination on intact Plasmodium falciparum-infected red blood cells (RBC) has identified a 125I-labelled antigen with an apparent size of about 155 kD. This labelled protein was specifically immunoprecipitated by the following antibodies: a rabbit antiserum and a mouse monoclonal antibody raised against a synthetic peptide comprising the 3',8-mer repeat EENVEHDA of the Pf155/RESA protein; a rabbit antiserum raised against a synthetic octapeptide comprising two copies of the 3',4-mer repeat EENV of the Pf155/RESA protein; and rabbit antisera against another synthetic peptide C(MYSNNNVED)2. The last antibody shows a strong reaction in asexual blood stage parasites with the Pf155/RESA antigen. While this antigen has been described previously as a submembrane component of the outer membrane of infected RBC, this report shows that at least part of its is accessible to the surface of both ring and late trophozoite-infected erythrocytes. |
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ISSN: | 0818-9641 1440-1711 |
DOI: | 10.1038/icb.1988.36 |