Kelakai Extract Protects Skin From UV-Induced Oxidative Damage

• Published in: Journal of physics. Conference series Vol. 1374; no. 1; pp. 12014 - 12019
• Main Authors: Mashuri, M, Sihombing, L D M, Alfaqihah, S, Edyson, E, Suhartono, E
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.11.2019
• Subjects: Anions; Antioxidants; Carbonyls; Damage; Ethanol; Exposure; Oxidation; Oxidative stress; Oxidizing agents; Oxygen compounds; Physics; Random sampling; Rats; Sampling methods; Statistical methods; Superoxide dismutase
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1374/1/012014

Oxidative stress is an unbalance situation between oxidant and antioxidant. Oxidative stress can be caused by UV exposure until it can result skin damage. This damage caused by the production of reactive oxygen compound (ROC), such as excessive superoxide anion. Thus, natural exogen antioxidant is needed, that is Kelakai. This research is pure experimental research with rats (Rattus norvegicus) as its subject. There were 24 samples were divided into 4 treatment groups; samples were chosen used simple random sampling method and repetition value used Federe method. Kelakai leaves were extracted using ethanol with maseration method. Superoxide anion level and superoxide dismutase enzyme activities were measured with Misra and Fridovich method; carbonyl level was measured using modified DNPH (dinitro phenilhidrazin) method; and conjugated diena level was measured with Kwiat Kowska method. The data was analysed statistically, and resulted significant differences between the group that only exposed to UV and the group that was given Kelakai extract and exposed to UV. Based on that result, it can be concluded that Kelakai extract can influence superoxide dismutase activities, superoxide anion level, carbonyl level, and conjugated diena in rats skin exposed to UV.