Multimodal approach to reveal the effect of light irradiation on chemical composition of lipofuscin granules of human RPE tissues

The accumulation of lipofuscin (LF) in tissues is considered as one a hallmark of the aging process. Usually formed in yellow-brown pigment aggregates or granules primarily composed of lipid residues and proteins, the exact chemical composition of LF varies among tissues. LF of the retinal pigment e...

Full description

Saved in:
Bibliographic Details
Published inJournal of physics. Conference series Vol. 1695; no. 1; pp. 12063 - 12069
Main Authors Aybush, Arseny V, Gulin, Alexander A, Vasin, Alexander A, Dontsov, Alexander E, Nadtochenko, Victor A, Ostrovsky, Mikhail A
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.12.2020
Subjects
Age related diseases
Bioaccumulation
Broadband
Chemical composition
Chemical compounds
Eye diseases
Fluorescence
Granular materials
Light irradiation
Lipids
Photochemistry
Physics
Toxicity
Online AccessGet full text

Cover

More Information
Summary:The accumulation of lipofuscin (LF) in tissues is considered as one a hallmark of the aging process. Usually formed in yellow-brown pigment aggregates or granules primarily composed of lipid residues and proteins, the exact chemical composition of LF varies among tissues. LF of the retinal pigment epithelium (RPE) is known to contain byproducts of the visual cycle, including one of the fluorophores in LF bis-retinoid N-retinyl-N-retinylidene ethanolamine (A2E). The last is characterized by high photoreactivity and implicates in age-related macular degeneration. Photoinduced production of reactive oxygen species (ROS) is shown among the main modes of A2E toxicity although particular photochemistry of A2E have yet to be completely identified. In this work we study changes in chemical composition of LF granules of human RPE under light irradiation using vibrational spectroscopy (femtosecond broadband CARS) and mass-spectrometry (TOF-SIMS) approach accompanied by measurements of fluorescence properties of the LF.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1695/1/012063