Correlation between human sperm hypo-osmotic swelling test and other semen parameters

• Published in: Journal of clinical laboratory analysis Vol. 7; no. 4; pp. 243 - 246
• Main Authors: Al-Mogazy, Hassan M., Al-Khadra, Samih, Kenawy, Gaafar, Al-Zeftawy, Ali, Mostafa, Taymour, Attia, Ahmed, Attia, Abdalla M.
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 1993
• Subjects: Adult; hos-test; Humans; Infertility, Male - diagnosis; Male; male infertility; Middle Aged; Osmotic Pressure; semen; specific gravity; Sperm Count; Sperm Motility; Spermatozoa - physiology; total protein
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.1860070410

Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo‐osmotic swelling test was done for all these samples, measuring total protein and specific gravity. A significant positive correlation was observed between percent sperm swelling and its motility, its total protein, and specific gravity. Also a significant negative correlation was noticed between percent sperm swelling and its count. Although there were overlapping ranges, different groups showed significant relations of percent sperm swelling among themselves. It is postulated that the sperm quality and not its quantity is the cornerstone of the hypoosmotic test and that the physico‐chemical properties of the sperm influences its swelling. © 1993 Wiley‐Liss, Inc.