Expression and Purification of Recombinant Superoxide Dismutase (PaSOD) from Psychromonas arctica in Escherichia coli

• Published in: Bulletin of the Korean Chemical Society Vol. 32; no. 7; pp. 2405 - 2409
• Main Authors: Im, Ha-Na, Lee, Kyung-Hee
• Format: Journal Article
• Language: English
• Published: 대한화학회 20.07.2011
• Subjects: 화학
• ISSN: 0253-2964; 1229-5949
• DOI: 10.5012/bkcs.2011.32.7.2405

The psychrophilic bacteria Psychromonas arctica survives at subzero temperatures by having adapted several protective mechanisms against freezing and oxidative stresses. Many reactive oxygen species are likely generated in P. arctica as a result of reduced metabolic turnover rates. A previous study identified the pasod gene for superoxide dismutase from P. arctica using a series of PCR amplifications. Here, upon cloning into a His-tag fused plasmid, the sod gene from P. arctica (pasod) was successfully expressed by IPTG induction. His-tagged PaSOD was subsequently purified by Ni^(2+)-NTA affinity chromatography. The purified PaSOD exhibited a higher SOD activity than that of Escherichia coli (EcSOD) at all temperatures. The difference in activity between PaSOD and EcSOD becomes even more significant at 4 ^oC, indicating that PaSOD plays a functional role in the cold adaptation of P. arctica in the Arctic. KCI Citation Count: 4