In vitro trypanocidal activity of dibutyltin dichloride and its fatty acid derivatives

• Published in: Parasitology research (1987) Vol. 91; no. 1; pp. 5 - 11
• Main Authors: SHUAIBU, M. N, KANBARA, H, YANAGI, T, ICHINOSE, A, AMEH, D. A, BONIRE, J. J, NOK, A. J
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.09.2003
• Subjects: Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiparasitic agents; Biological and medical sciences; Fatty Acids - chemistry; Medical sciences; Mice; Microscopy, Electron; Microscopy, Electron, Scanning; Organotin Compounds - chemistry; Organotin Compounds - pharmacology; Parasitic Sensitivity Tests; Pharmacology. Drug treatments; Trypanocidal Agents - pharmacology; Trypanosoma - drug effects; Trypanosoma - growth & development; Trypanosoma - ultrastructure; Trypanosoma brucei brucei - drug effects; Trypanosoma brucei brucei - growth & development; Trypanosoma brucei brucei - ultrastructure; Trypanosoma brucei gambiense - drug effects; Trypanosoma brucei gambiense - growth & development; Trypanosoma brucei gambiense - ultrastructure; Trypanosoma brucei rhodesiense - drug effects; Trypanosoma brucei rhodesiense - growth & development; Trypanosoma brucei rhodesiense - ultrastructure; Kinetoplastida; Protozoa; Host parasite relation; Scanning electron microscopy; Protozoal disease; Metabolite; Parasite; Lipids; Parasitosis; African sickness; Fatty acids; In vitro; Survival; Biological activity; Infection; Antiprotozoal agent; Transmission electron microscopy; Ultrastructure; Morphology; Parasiticide; Trypanosoma brucei; Trypanosomiasis; Organic compounds
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-003-0861-2

Searching for new compounds against pathogenic trypanosomes has been substantially accelerated by the development of in vitro screening assays. In an attempt to explore the chemotherapeutic potential of organotin compounds and to broaden the search for newer trypanocides, fatty acid derivatives of dibutyltin dichloride were synthesized and their in vitro trypanocidal profiles studied on Trypanosoma brucei brucei, Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense. A 24-h time course experiment was conducted with various concentrations of the compounds using a 24-well microtiter plate technique. The compounds tested were trypanocidal in a dose-dependent fashion: inhibiting survival and growth, resulting in irreversible morphological deformation and the eventual death of the parasites. The minimum inhibitory concentrations of the tested diorganotins are at low micromolar ranges: from 0.15-0.75 microM for T. b. brucei, T. b. gambiense and T. b. rhodesiense. These observations suggest that organotin has chemotherapeutic potential.