A novel fluorescent probe based on naphthimide for H2S identification and application

• Published in: Analytical biochemistry Vol. 677; p. 115232
• Main Authors: Zhang, Cheng-lu, Liu, Chang, Ding, Yan-wei, Wang, Hai-tao, Nie, Shi-ru, Zhang, Yan-peng
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.09.2023
• Subjects: Fluorescent probe; Hydrogen sulfide; Naphthalimide; Selenoether bond; Hydrogen sulfide; Naphthalimide; Selenoether bond; Fluorescent probe
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2023.115232

In view of the superior chemical activity of selenoether bond (–Se–) and the excellent optical properties of naphthimide, a novel fluorescent probe (NapSe) with near-rectangular structure, which contains double naphthimide fluorophores linked by selenoether bond, is designed for specific fluorescence detection of hydrogen sulfide (H2S). NapSe has excellent optical properties: super large Stokes Shift (190 nm) and good stability in a wide pH range. The selectivity of NapSe fluorescence detection of H2S is high, and displays excellent "turn-on" phenomenon and strong anti-interference. And the fluorescence intensity increased obviously, reaching 42 times. The time response of probe NapSe is very rapid (3 min) compared with other fluorescence probes that respond to H2S. It shows high sensitivity by calculating the detection limit (LOD) as low as 5.4 μM. Notably, the identification of H2S by probe NapSe has been successfully applied to the detection of test paper and the detection of exogenous and endogenous fluorescence imaging of MCF-7 breast cancer cells. In view of the superior chemical activity of selenoether bond (–Se–) and the excellent optical properties of naphthimide, a novel fluorescent probe (NapSe) with near-rectangular structure, which contains double naphthimide fluorophores linked by selenoether bond, is designed for specific fluorescence detection of hydrogen sulfide (H2S). [Display omitted] •The probe NapSe has a very large Stokes shift (190 nm).•The probes (NapSe) can analyze environmental samples and living cells containing H2S.•The detection limit of probe NapSe is low (5.4 μM), the response time is fast (3min).