On the Isolation of Mitochondria with High Respiratory Control from Guinea Pig Pancreas

• Published in: Journal of biochemistry (Tokyo) Vol. 63; no. 3; pp. 332 - 340
• Main Authors: HONJO, ICHIO, TAKASAN, HIDENARI, OZAWA, KAZUE
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.03.1968
• Subjects: Animals; Centrifugation; Edetic Acid - pharmacology; Glutamates - pharmacology; Guinea Pigs; Hydrogen-Ion Concentration; Methods; Microscopy, Electron; Mitochondria - analysis; Mitochondria - metabolism; Oxidative Phosphorylation; Oxygen Consumption; Pancreas - analysis; Pancreas - cytology; Pancreas - metabolism; Polarography; Proteins - analysis; RNA - analysis; Serum Albumin - pharmacology; Succinates - pharmacology
• ISSN: 0021-924X; 1756-2651; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a128779

The present results show the isolation method of mitochondria with high respiratory control from guinea pig pancreas which is comparable to those prepared from other tissues. Care was mainly taken to separate the brown pellets as quickly as possible, which were precipitated from pancreas homogenates between 2000×g for 7 min and 6000×g for 10 min. The mitochondrial fractions prepared by this method consisted primarily of mitochondria with high phosphorylating efficiencies and were much less contaminated by non-mitochondrial particles, especially zymogen, than those obtained by other procedures. The quality of the mitochondrial preparation is considered acceptable as a starting material for biochemical work.