Molecularly imprinted polymers applied to the clean-up of zearalenone and alpha-zearalenol from cereal and swine feed sample extracts

• Published in: Analytical and bioanalytical chemistry Vol. 385; no. 7; pp. 1155 - 1161
• Main Authors: Urraca, Javier L, Marazuela, María Dolores, Moreno-Bondi, María C
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01.08.2006
• Subjects: Acetic acid; Ammonium; Ammonium acetate; Animal Feed - standards; Animals; Corn; Edible Grain - standards; Estrogens, Non-Steroidal - isolation & purification; Flow velocity; Fluorescence; Food Contamination; High-performance liquid chromatography; Imprinted polymers; Liquid chromatography; Metabolites; Methods; Mimicry; Molecular Conformation; Mycotoxins; Polymers; Stationary phase; Swine; Zearalenone; Zearalenone - isolation & purification; Zeranol - analogs & derivatives; Zeranol - isolation & purification
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-006-0343-3

A molecularly imprinted polymer prepared using 1-allylpiperazine (1-ALPP) as the functional monomer, trimethyltrimethacrylate (TRIM) as the crosslinker and the zearalenone (ZON)-mimicking template cyclododecanyl-2,4-dihydroxybenzoate (CDHB) has been applied to the clean-up and preconcentration of this mycotoxin (zearalenone) and a related metabolite, alpha-zearalenol (alpha-ZOL), from cereal and swine feed sample extracts. The extraction of ZON and alpha-ZOL from the food samples was accomplished using pressurized liquid extraction (PLE) with MeOH/ACN (50:50, v/v) as the extraction solvent, at 50 degrees C and 1500 psi. The extracted samples were cleaned up and preconcentrated through the MIP cartridge and analyzed using HPLC with fluorescence detection (lambda (exc)=271/ lambda (em)=452 nm). The stationary phase was a polar endcapped C18 column, and ACN/MeOH/water 10/55/35 (v/v/v, 15 mM ammonium acetate) at a flow rate of 1.0 mL min(-1) was used as the mobile phase. The method was applied to the analysis of ZON and alpha-ZOL in wheat, corn, barley, rye, rice and swine feed samples fortified with 50, 100 and 400 ng g(-1) of both mycotoxins, and it gave recoveries of between 85 and 97% (RSD 2.1-6.7%, n=3) and 87-97% (RSD 2.3-5.6%, n=3) for alpha-ZOL and ZON, respectively. The method was validated using a corn reference material for ZON.