Quantifying protein in cerebrospinal fluid and urine: success achieved

The method involved coprecipitation of the proteins with a Ponceau S dye trichloroacetic acid solution, dissolving the protein precipitate in dilute sodium hydroxide, and measuring the absorbance at 560 nm. Sadly, in today's hospital environment, most of the laboratory director's time is c...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 54; no. 4; p. 770
Main Author Pesce, Michael A
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.04.2008
Subjects
Azo Compounds
Body fluids
Cerebrospinal Fluid Proteins - analysis
Chemistry
Coloring Agents
Dyes
History, 20th Century
Humans
Methods
Michael A Pesce
Microchemistry - history
Microchemistry - methods
Pesce
Proteins
Proteinuria - diagnosis
Urine
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Summary:The method involved coprecipitation of the proteins with a Ponceau S dye trichloroacetic acid solution, dissolving the protein precipitate in dilute sodium hydroxide, and measuring the absorbance at 560 nm. Sadly, in today's hospital environment, most of the laboratory director's time is consumed by administrative tasks, accreditation and state regulatory requirements, and budgetary obligations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Biography-3
content type line 23
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2007.094177