Bamboo Salt Suppresses Colon Carcinogenesis in C57BL/6 Mice with Chemically Induced Colitis

• Published in: Journal of medicinal food Vol. 19; no. 11; p. 1015
• Main Authors: Ju, Jaehyun, Lee, Ga-Young, Kim, Yoon-Se, Chang, Hee Kyung, Do, Myoung-Sool, Park, Kun-Young
• Format: Journal Article
• Language: English
• Published: United States 01.11.2016
• Subjects: Animals; Anticarcinogenic Agents - pharmacology; Apoptosis - drug effects; bcl-2-Associated X Protein; Carcinogenesis - drug effects; Carcinogenesis - metabolism; Carcinogenesis - pathology; Cell Transformation, Neoplastic - chemically induced; Cell Transformation, Neoplastic - drug effects; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - pathology; Colitis - chemically induced; Colitis - drug therapy; Colitis - genetics; Colitis - metabolism; Colon - anatomy & histology; Colon - drug effects; Colon - pathology; Colonic Neoplasms - blood; Colonic Neoplasms - genetics; Colonic Neoplasms - pathology; Colonic Neoplasms - prevention & control; Cytokines - blood; Disease Models, Animal; HT29 Cells; Humans; Immunohistochemistry; Male; Mice; Mice, Inbred C57BL; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Sodium Chloride, Dietary - pharmacology; dextran sodium sulfate; colon cancer; azoxymethane; bamboo salt
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2016.3798

The aim of our experiment was to evaluate the anticancer effect of bamboo salt (BS) on C57BL/6 mice in an azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon cancer model. BS, solar salt, and purified salt were evaluated for their protective effects during AOM/DSS-induced colon carcinogenesis in C57BL/6 mice. BS, especially after baking for nine separate intervals (BS9x), suppressed colon carcinogenesis in the mice. BS9x decreased colon length shortening, weight-to-length ratios, and tumor counts. Pathological evidence from histological evaluation by hematoxylin and eosin staining also revealed suppression of tumorigenesis. BS9x lowered serum levels of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) to close to those of the Normal group. Additionally, BS9x suppressed colon mRNA expression of proinflammatory factors and significantly regulated mRNA levels of the apoptosis-related factors, Bax and Bcl-2, and the cell cycle-related genes, p21 and p53. Additionally, immunohistochemistry showed that BS promoted p21 expression in the colon. Taken together, the results indicate that BS exhibited anticancer efficacy by modulating apoptosis- and inflammation-related gene expression during colon carcinogenesis in mice, and repetition in baking cycles of BS enhanced its anticancer functionality.