Radioisotope dilution technique for determination of vitamin B12 in foods
A radioisotope dilution (RID) method for the determination of vitamin B12 is presented. The method combines a standard extraction procedure (AOAC 43.108, 12th ed.) with a commercially available RID assay kit. The method was evaluated on a wide range of fortified and unfortified food products. Recove...
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Published in | Journal of the Association of Official Analytical Chemists Vol. 65; no. 1; p. 85 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.01.1982
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Subjects | |
Online Access | Get more information |
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Summary: | A radioisotope dilution (RID) method for the determination of vitamin B12 is presented. The method combines a standard extraction procedure (AOAC 43.108, 12th ed.) with a commercially available RID assay kit. The method was evaluated on a wide range of fortified and unfortified food products. Recovery studies on both groups yielded average recoveries of 98.1 and 95.8%, respectively. Reproducibility data generated from replicate analyses on both groups gave a relative standard deviation of 6.9% for the fortified group and 9.2% for the unfortified group. For the samples studied, the mean vitamin B12 content determined by the RID method was 8.01 micrograms/100 g vs a mean of 7.54 micrograms/100 g by the AOAC microbiological method; the correlation coefficient was r = 0.983. |
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ISSN: | 0004-5756 |
DOI: | 10.1093/jaoac/65.1.85 |