An artificial blood vessel with an endothelial-cell monolayer

An artificial blood vessel with an endothelial-cell monolayer was used as an arterial substitute in rats. Endothelial cells were isolated from the aorta of a Wistar rat by the digestion method. The cell identification was established by the cobblestone appearance of a confluent cell monolayer, by an...

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Bibliographic Details
Published inJournal of neurosurgery Vol. 77; no. 3; p. 397
Main Authors Kobayashi, H, Kabuto, M, Ide, H, Hosotani, K, Kubota, T
Format Journal Article
LanguageEnglish
Published United States 01.09.1992
Subjects
Animals
Aorta - cytology
Blood Vessel Prosthesis - methods
Carotid Arteries - surgery
Cells, Cultured
Chi-Square Distribution
Endothelium, Vascular - cytology
Male
Polytetrafluoroethylene
Rats
Rats, Inbred Strains
Vascular Patency
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Summary:An artificial blood vessel with an endothelial-cell monolayer was used as an arterial substitute in rats. Endothelial cells were isolated from the aorta of a Wistar rat by the digestion method. The cell identification was established by the cobblestone appearance of a confluent cell monolayer, by an expression of factor VIII-related antigen, and by the presence of Weibel-Palade bodies. The luminal surface of the thin-walled polytetrafluoroethylene (PTFE) graft (4 mm in diameter and 10 mm in length) was coated with an endothelial-cell monolayer for 7 days in vitro. An interpositional graft was placed using the endothelial cell-coated PTFE prosthesis on the right common carotid artery in seven rats. A total of 10 rats received an interpositional graft with the noncoated PTFE prosthesis as a control. The patency rate at 1 month after implantation was significantly higher in the coated group than in the control group. The vascular prosthesis with an endothelial-cell monolayer is a promising technique to inhibit the development of thrombosis.
ISSN:0022-3085
DOI:10.3171/jns.1992.77.3.0397