Molecular identification of Trichinella spiralis and Trichinella britovi by diagnostic multiprimer large mitochondrial rRNA amplification

• Published in: Parasitology research (1987) Vol. 91; no. 5; pp. 374 - 377
• Main Authors: BORSUK, P, MOSKWA, B, PASTUSIAK, K, CABAJ, W
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.11.2003
• Subjects: Animals; Animals, Wild - parasitology; Base Sequence; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Invertebrates; Molecular Epidemiology - methods; Molecular Sequence Data; Nemathelminthia. Plathelmintha; Poland; Polymerase Chain Reaction - veterinary; RNA - genetics; RNA, Helminth - genetics; Swine - parasitology; Swine Diseases - parasitology; Systematics. Geographical distribution; Trichinella spiralis - classification; Trichinella spiralis - genetics; Trichinella spiralis - isolation & purification; Trichinellosis - parasitology; Trichinellosis - veterinary; Poland; Host parasite relation; Methodology; Nucleotide sequence; Taxonomy; Chemotaxonomy; Parasite; Mitochondrial DNA; Parasitosis; Method; Helminthiasis; Infection; Ribosomal RNA; Nematode disease; Domestic animal; Helmintha; Genetic identification; Nemathelminthia; Specific identification; Trichinella spiralis; Diagnosis; Invertebrata; Nematoda; Trichinosis
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-003-0971-x

Trichinella parasites with different epidemiological features still occur in Europe and four species of genus Trichinella have been identified: T. spiralis, T. britovi, T. nativa and T. pseudospiralis. Until now, two of them, T. spiralis and T. britovi, have been identified in Poland. In our studies we selected sequence coding for large mitochondrial rRNA (mt LrDNA) as a genetic marker and developed a sensitive LrDNA multiprimer PCR assay allowing for rapid identification of T. spiralis and T. britovi, parasites present in wild and domestic animals in Poland.