Ultrasonic-augmented Primary Adult Fibroblast Isolation

• Published in: Journal of visualized experiments no. 149
• Main Authors: Künzel, Stephan R, Schaeffer, Charlotte, Sekeres, Karolina, Mehnert, Carola S, Schacht Wall, Stephanie M, Newe, Manja, Kämmerer, Susanne, El-Armouche, Ali
• Format: Journal Article
• Language: English
• Published: United States 29.07.2019
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/59858

Primary adult fibroblasts have become an important tool to study fibrosis, fibroblast interactions and inflammation in all body tissues. Since primary fibroblasts cannot divide indefinitely due to myofibroblast differentiation or senescence induction, new cultures must be established regularly. However, there are several obstacles to overcome during the processes of developing a reliable isolation protocol and primary fibroblast isolation itself: the method's degree of difficulty (especially for beginners), the risk of bacterial contamination, the required time until primary fibroblasts can be used for experiments, and subsequent cell quality and viability. In this study, a fast, reliable and easy-to-learn protocol to isolate and culture primary adult fibroblasts from mouse heart, lung, liver and kidney combining enzymatic digestion and ultrasonic agitation is provided.