A novel approach for amine derivatization of MoS2 nanosheets and their application toward label-free immunosensor
The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In...
Saved in:
Published in | Analytical biochemistry Vol. 555; pp. 1 - 8 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
15.08.2018
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS2 nanosheets. This newly proposed approach helped to immobilize the MoS2 nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS2 nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS2 nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the ‘prostate specific antigen’. The application of the proposed immunosensor was characterized with a low detection limit (10−3 ng/mL) over a very wide quantitation range (10−3 to 200 ng/mL).
•The bioconjugation of MoS2 nanosheets has been processed with a novel strategy.•MoS2 nanosheets were exfoliated and electrochemically modified with 2-aminobenzylamine.•MoS2 nanosheets have been interfaced with antibodies via facile EDC/NHS cross-linking chemistry.•MoS2 nanosheets immunosensor for PSA has offered a low detection limit and a very wide range of analysis. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/j.ab.2018.05.029 |