Simultaneous quantification of three isoflavonoid glycosides in rabbit plasma after oral administration of Astragalus mongholicus extract by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

• Published in: Analytica chimica acta Vol. 602; no. 2; pp. 252 - 258
• Main Authors: Zhang, X., Sun, Y.G., Cheng, M.C., Wang, Y.Q., Xiao, H.B., Liang, X.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.10.2007; Elsevier
• Subjects: Administration, Oral; Analytical chemistry; Animals; Asparagus Plant - chemistry; Astragalus mongholicus; Calibration; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Exact sciences and technology; Glycosides - blood; Isoflavones - blood; Isoflavonoid glycoside; Liquid chromatography; Molecular Structure; Onions - chemistry; Other chromatographic methods; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plasma; Rabbits; Spectrometric and optical methods; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry; Astragalus mongholicus; Tandem mass spectrometry; Plasma; Liquid chromatography; Isoflavonoid glycoside; Solid phase extraction; Chemical analysis; Internal standard; HPLC chromatography; Rabbit; Electrospray; Chemical enrichment; Sample preparation; Detection limit; Standard curve; Glycoside; Monitoring; Trace analysis; Correlation coefficient; Linear regression; Lagomorpha; Chemical ionization; Vertebrata; Mammalia; Mass spectrometry MS/MS; Simultaneous measurement; Reference material; Pharmacokinetics
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2007.09.033

A selective and sensitive liquid chromatography coupled with triple stage quadruple tandem mass spectrometry (HPLC/TSQ-MS/MS) was developed and validated for simultaneous quantification of calycosin-7- O-β- d-glycoside (CCSG), formononetin-7- O-β- d-glycoside (Ononin) and ( 6R, 10R)-9,10-dimethoxypterocarpan-3- O-β- d-glycoside (DPG) in rabbit plasma. Plasma samples were extracted with solid-phase extraction (SPE), separated on an Inertsil ODS-3 column and detected by tandem mass spectrometry with electrospray ionization (ESI) interface in positive selective reaction monitoring (SRM) mode. 3,7,8-Trimethoxy-xanthone-1- O-primaverose was used as internal standard (IS) for quantitative measurement. For each analyte, one major product ion was chosen and used for screening of it. Calibration curves were generated over the range of 2–1000 ng mL −1 with the correlation coefficients greater than 0.99 by using a weighted (1/ χ) least squares linear regression. The method had the lower limit quantification of 0.15, 0.21 and 0.19 for CCSG, Ononin and DPG, respectively, with precision less than 20%. The intra- and inter-day precisions ranged from 2.48 to 6.38% and 4.81 to 11.78% (R.S.D.%), respectively. This assay is suitable for determining the above three trace glycosides in rabbit plasma simultaneously and thus investigating the pharmacokinetics of glycosides from Astragalus mongholicus extract in rabbits.