Functional genomics guided with MR imaging: mouse tumor model study

To gain a better understanding of gene expression patterns in tumors, the authors used contrast material-enhanced magnetic resonance (MR) imaging to noninvasively characterize regions within the same tumor to provide a correlate for genomic analysis. Gene expression profiles of samples from a mouse...

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Bibliographic Details
Published inRadiology Vol. 228; no. 2; p. 560
Main Authors Guccione, Samira, Yang, Yi-Shan, Shi, Gongyi, Lee, Daniel Y, Li, King C P, Bednarski, Mark D
Format Journal Article
LanguageEnglish
Published United States 01.08.2003
Subjects
Animals
Blotting, Western
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Contrast Media
Disease Progression
Gadolinium DTPA
Gene Expression Profiling
Genomics
Magnetic Resonance Imaging - methods
Mice
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
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Summary:To gain a better understanding of gene expression patterns in tumors, the authors used contrast material-enhanced magnetic resonance (MR) imaging to noninvasively characterize regions within the same tumor to provide a correlate for genomic analysis. Gene expression profiles of samples from a mouse tumor model obtained from contrast-enhanced and nonenhanced regions within the same tumor were compared with MR imaging and functional genomics. From these samples, 11000 genes were analyzed: 10 genes were up-regulated in the contrast-enhanced areas, and one gene was up-regulated in the nonenhanced regions. Several of these genes encode extracellular matrix proteins. Findings in this study demonstrate that MR imaging can serve as a powerful noninvasive tool for characterizing different regions of tumors to guide genomic analysis with high spatial and temporal resolution.
ISSN:0033-8419
DOI:10.1148/radiol.2282020907