Fabrication of Biofunctionalized Quasi-Three-Dimensional Microstructures of a Nonfouling Comb Polymer Using Soft Lithography
This paper describes a simple set of patterning methods that are applicable to diverse substrates and allow the routine and rapid fabrication of protein patterns embedded within a background that consists of quasi‐three‐dimensional microstructures of a cell‐resistant polymer. The ensemble of methods...
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Published in | Advanced functional materials Vol. 15; no. 4; pp. 529 - 540 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.04.2005
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | This paper describes a simple set of patterning methods that are applicable to diverse substrates and allow the routine and rapid fabrication of protein patterns embedded within a background that consists of quasi‐three‐dimensional microstructures of a cell‐resistant polymer. The ensemble of methods reported here utilizes three components to create topographically nonfouling polymeric structures that present cell‐adhesive protein patterns in the regions between the microstructures: the first component is an amphiphilic comb polymer that is comprised of a methyl methacrylate backbone and pendant oligo(ethylene glycol) moieties along the side chain, physically deposited films of which are protein‐ and cell‐resistant. The second component of the fabrication methodology involves the use of different variants of soft lithography, such as microcontact printing to create nonfouling topographical features of the comb polymer that demarcate cell‐adhesive regions of the third component: a cell‐adhesive extracellular protein or peptide. The ensemble of methods reported in this paper was used to fabricate quasi‐three‐dimensional patterns that present topographical and biochemical cues on a variety of substrates, and was shown to successfully maintain cellular patterns for up to two months in serum‐containing medium. We believe that this, and other such methods under development that allow independent and systematic control of chemistry, topography and substrate compliance will provide versatile “test‐beds” for fundamental studies in cell biology as well as allow the discovery of rational design principles for the development of biomaterials and tissue‐engineering scaffolds.
A simple set of methods for the fabrication of quasi‐3D patterns that present topographical and biochemical cues to cells (see Figure) on a variety of substrates are presented. These methods are shown to successfully maintain cellular patterns for up to two months in serum‐containing media. |
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Bibliography: | ark:/67375/WNG-7PGMV86L-Z istex:17EFB736CFB0CF8B325D776BE3E86377EC92F784 The authors thank Mr. Yong Wang for help with the viscosity measurements. This work was partially supported by the Center for Biologically Inspired Materials and Materials System (CBIMMS) at Duke University and by the NSF through grant EEC-0210590 to A. C. ArticleID:ADFM200400088 The authors thank Mr. Yong Wang for help with the viscosity measurements. This work was partially supported by the Center for Biologically Inspired Materials and Materials System (CBIMMS) at Duke University and by the NSF through grant EEC‐0210590 to A. C. |
ISSN: | 1616-301X 1616-3028 |
DOI: | 10.1002/adfm.200400088 |