Proteomic analysis of Staphylococcus aureus exposed to bacteriocin XJS01 and its bio-preservative effect on raw pork loins

• Published in: Meat science Vol. 204; p. 109258
• Main Authors: Ying, Jian-Ping, Wu, Gang, Zhang, Yan-Mei, Zhang, Qi-Lin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2023
• Subjects: Antibacterial mechanism; Bacteriocin XJS01; Preserving effect; Raw pork loins; Staphylococcus aureus; TMT proteomics; Raw pork loins; TMT proteomics; Preserving effect; Bacteriocin XJS01; Antibacterial mechanism; Staphylococcus aureus
• ISSN: 0309-1740; 1873-4138
• DOI: 10.1016/j.meatsci.2023.109258

Antibacterial mechanism of bacteriocins against foodborne S. aureus is still to be explored, particularly in proteomics, and a deep and comprehensive study on application of bacteriocins for preservation of raw pork is required. Here, proteomic mechanism of Lactobacillus salivarius bacteriocin XJS01 against foodborne S. aureus 2612:1606BL1486 (S. aureus_26) and its preservation effect on raw pork loins stored at 4 °C for 12 days was investigated. The results showed that 301 differentially abundant proteins (DAPs) were identified between XJS01-treated and -free groups (control group) using Tandem mass tag (TMT) quantitative proteomics technology, which were primarily involved in amino acids and carbohydrate metabolism, cytolysis, defense response, cell apoptosis, cell killing, adhesion, and oxygen utilization of S. aureus_26. Bacterial secretion system (SRP) and cationic antimicrobial peptide resistance may be key pathways to maintain protein secretion and counteract the deleterious effects on S. aureus_26 caused by XJS01. In addition, XJS01 could significantly improve the preservation of raw pork loins by the evaluation results of sensory and antibacterial activity on the meat surface. Overall, this study showed that XJS01 induced a complex organism response in S. aureus, and it could be potential pork preservative. •Bacteriocin XJS01 elicit proteomic response involved in metabolism, cytolysis, and defense response in S. aureus.•SRP and CAMP resistance pathways are key targets of action of XJS01.•XJS01 significantly improved the preservation of raw pork loins.•XJS01 could be a potential biocontrol agent on pork.