Facile construction of Mo-based nanozyme system via ZIF-8 templating with enhanced catalytic efficiency and antibacterial performance

• Published in: Heliyon Vol. 10; no. 18; p. e38057
• Main Authors: Jia, Haoruo, Zheng, Ziyuan, Qu, Jining, Yu, Hongtao, Zhu, Zhoujun, Lu, Qingda, Su, Fei, Yang, Yating, Feng, Tongtong, Jie, Qiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.09.2024; Elsevier
• Subjects: Antibacterial activity; Enzymatic cascade; Molybdenum; Single-atom nanozymes; Single-atom nanozymes; Antibacterial activity; Enzymatic cascade; Molybdenum
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e38057

Although Zeolitic Imidazolate Framework-8 (ZIF-8) shows significant promise in chemodynamic therapy of bacterial infections due to its large specific surface area and enzyme-like activity, it still faces a considerable gap compared to natural enzymes. The dependency on low pH and high concentrations of hydrogen peroxide ((H2O2) is a major factor limiting the clinical progress of nanozymes. Single-atom nanozymes (SA-zyme), which exhibit superior catalytic performance, are expected to overcome this limitation. In this study, we used ZIF-8 as a template to prepare structurally regular molybdenum-based single-atom nanozymes (Mo-zyme) by coordinating molybdenum atoms with nitrogen atoms within the zeolitic imidazolate framework and evaporating the zinc element at high temperatures. The cascade catalytic performance of the nanodrugs was enhanced by loading glucose oxidase (GOx) and encapsulating it with a hyaluronic acid (HA) layer to form a composite (Mo/GOx@HA). Upon contact with hyaluronidase from bacteria in infected tissues, the cascade reaction is triggered, resulting in the degradation of the HA shell, and releasing the encapsulated GOx. Once exposed, GOx catalyzes the oxidation of glucose into gluconic acid, resulting in a localized decrease in pH and continuous production of H2O2. The combination of lowered pH and increased H2O2 concentration significantly amplifies the catalytic activity of the Mo-zyme. This enhanced activity facilitates the in situ generation of hydroxyl radicals (•OH) on the bacterial surface, leading to effective and efficient bacterial eradication. Wound infection treatment has demonstrated that the as-prepared Mo/GOx@HA exhibits excellent antibacterial and anti-inflammatory activity. This work provided a promising enzymatic cascade reaction nanoplatform for the treatment of bacteria infected wounds. The illustration of cascade catalytic reaction as self-activating nanoreactor for antibacterial. [Display omitted] •Mo-zymewith a regular morphology is successfully prepared through pyrolysis, ensuring the Mo atoms are well dispersed within the ZIF-8 precursor framework.•Mo/GOx@HA is triggered by hyaluronidase derived from bacterial secretion, initiating a targeted antibacterial response.•The Mo/GOx@HA system overcomes the limitations of pH and H2O2 dependence, providing a more versatile and effective antibacterial process.