Unravelling pine response to Fusarium circinatum through Raman spectroscopy

• Published in: Journal of Raman spectroscopy Vol. 53; no. 12; pp. 2086 - 2099
• Main Authors: Martín, Daniel, Santos, Inês P., Monteiro, Pedro, Amaral, Joana, Costa, Ricardo M. F., Martín‐Garcia, Jorge, Batista de Carvalho, Luís A. E., Marques, Maria Paula M., Pinto, Glória
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 01.12.2022
• Subjects: Cell walls; Cost analysis; Disease control; Disease detection; Ecological effects; Fusarium; Fusarium circinatum; Inoculation; Necrosis; Phenolic compounds; Phenols; Pine; pine pitch canker; Pine trees; Pinus pinea; Pinus radiata; Pinus spp; Pitch canker; plant protection; Raman spectroscopy; Signs and symptoms; species selection; Spectroscopy; Spectrum analysis
• ISSN: 0377-0486; 1097-4555
• DOI: 10.1002/jrs.6446

Pine Pitch Canker (PPC), caused by the fungus Fusarium circinatum, is associated to significant economic and ecological losses worldwide. The effectiveness of PPC monitoring, early detection in nurseries and plantations, and the identification of resistant plant material relies on the development of objective, non‐destructive, and cost‐effective tools. This study analyzed the potential of employing Raman Spectroscopy (RS) for the early detection of biochemical changes associated with PPC in Pinus spp. with different susceptibilities to F. circinatum (highly susceptible Pinus radiata vs relatively resistant Pinus pinea), while unveiling possible mechanisms of action on these pathosystems. Our results indicate lignin as a key constitutive component of pine resistance against PPC and thus the potential of using this technology for the selection of PPC resistant trees. Moreover, we demonstrate the power of RS‐based approaches for the rapid detection of the disease in susceptible species. Early spectral variations were found in P. radiata upon inoculation with F. circinatum from 3 days post‐inoculation (dpi) onwards, whereas changes in histological analysis, relative internal stem necrosis measurements, and visual disease symptoms were only visible at 6, 8, and 9 dpi, respectively. These spectral changes have been associated to cell wall degradation and induction of phenolic compounds synthesis upon infection in P. radiata. Altogether, we believe that RS is an innovative promising tool able to reduce disease detection time in pine species and providing an appealing alternative for the development of new and eco‐friendly disease control measures. Pine Pitch Canker (PPC), caused by the fungus Fusarium circinatum, represents high‐impact economic and ecological losses worldwide. This study analyzed the potential of Raman Spectroscopy (RS) for an early detection of biochemical changes associated with PPC. The results demonstrate the ability of RS to detect early spectral variations in Pinus radiata upon inoculation with Fusarium circinatum at 3 days post‐inoculation, whereas changes were visible only at 6–8 days after inoculation through other methods. This represents a new promising tool for the development of new and eco‐friendly disease control measures.